Resolvin D2 uses multiple Ca²⁺ -dependent signaling pathways to stimulate mucin secretion in rat and human conjunctival goblet cells.

ABSTRACT: The mucin layer of the tear film is produced by goblet cells in the conjunctiva to protect the ocular surface and maintain homeostasis. The pro-resolving lipid mediator resolvin D2 (RvD2) biosynthesized from an omega 3 fatty acid actively terminates inflammation and regulates mucin secretion from conjunctival goblet cells. Our objective was to determine which Ca²⁺ -dependent signaling pathways RvD2 uses to stimulate conjunctival goblet cell function (CGC). We hypothesize that RvD2 activates multiple intracellular Ca²⁺ signaling pathways to stimulate CGC secretion. Rat and human CGCs were cultured from conjunctival explants. The amount of RvD2 receptor GPR18/DRV2 message and protein were determined. The intracellular concentration of Ca²⁺ ([Ca²⁺ ]

SUBMITTER: Botten N 

PROVIDER: S-EPMC9560994 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature