Generalizable strategy to analyze domains in the context of parent protein architecture: A CheW case study.

ABSTRACT: Domains are the three-dimensional building blocks of proteins. An individual domain can occur in a variety of domain architectures that perform unique functions and are subject to different evolutionary selective pressures. We describe an approach to evaluate the variability in amino acid sequences of a single domain across architectural contexts. The ability to distinguish different evolutionary outcomes of one protein domain can help determine whether existing knowledge about a specific domain will apply to an uncharacterized protein, lead to insights and hypotheses about function, and guide experimental priorities. We developed and tested our approach on CheW-like domains (PF01584), which mediate protein/protein interactions and are difficult to compare experimentally. CheW-like domains

SUBMITTER: Vass LR 

PROVIDER: S-EPMC9561059 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature