Continuous biomarker monitoring with single molecule resolution by measuring free particle motion.

ABSTRACT: There is a need for sensing technologies that can continuously monitor concentration levels of critical biomolecules in applications such as patient care, fundamental biological research, biotechnology and food industry, as well as the environment. However, it is fundamentally difficult to develop measurement technologies that are not only sensitive and specific, but also allow monitoring over a broad concentration range and over long timespans. Here we describe a continuous biomolecular sensing methodology based on the free diffusion of biofunctionalized particles hovering over a sensor surface. The method records digital events due to single-molecule interactions and enables biomarker monitoring at picomolar to micromolar concentrations without consuming any reagents. We demonstrate the

SUBMITTER: Buskermolen AD 

PROVIDER: S-EPMC9561105 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature