Traceless cysteine-linchpin enables precision engineering of lysine in native proteins.

ABSTRACT: The maintenance of machinery requires its operational understanding and a toolbox for repair. The methods for the precision engineering of native proteins meet a similar requirement in biosystems. Its success hinges on the principles regulating chemical reactions with a protein. Here, we report a technology that delivers high-level control over reactivity, chemoselectivity, site-selectivity, modularity, dual-probe installation, and protein-selectivity. It utilizes cysteine-based chemoselective Linchpin-Directed site-selective Modification of lysine residue in a protein (LDM_C-K). The efficiency of the end-user-friendly protocol is evident in quantitative conversions within an hour. A chemically orthogonal C-S bond-formation and bond-dissociation are essential among multiple regul

SUBMITTER: Reddy NC 

PROVIDER: S-EPMC9561114 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature