Protein delivery to living cells by thermal stimulation for biophysical investigation.

ABSTRACT: Studying biomolecules in their native environment represents the ideal sample condition for structural biology investigations. Here we present a novel protocol which allows to delivery proteins into eukaryotic cells through a mild thermal stimulation. The data presented herein show the efficacy of this approach for delivering proteins in the intracellular environment of mammalian cells reaching a concentration range suitable for successfully applying biophysical methods, such as double electron electron resonance (DEER) measurements for characterising protein conformations.

SUBMITTER: Torricella F 

PROVIDER: S-EPMC9561116 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature