CRISPRactivation-SMS, a message for PAM sequence independent gene up-regulation in Escherichia coli.

ABSTRACT: Governance of the endogenous gene regulatory network enables the navigation of cells towards beneficial traits for recombinant protein production. CRISPRactivation and interference provides the basis for gene expression modulation but is primarily applied in eukaryotes. Particularly the lack of wide-ranging prokaryotic CRISPRa studies might be attributed to intrinsic limitations of bacterial activators and Cas9 proteins. While bacterial activators need accurate spatial orientation and distancing towards the target promoter to be functional, Cas9-based CRISPR tools only bind sites adjacent to NGG PAM sequences. These circumstances hampered Cas9-guided activators from mediating the up-regulation of endogenous genes at precise positions in bacteria. We could overcome this limitation by combin

SUBMITTER: Klanschnig M 

PROVIDER: S-EPMC9561276 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature