Genetic context effects can override canonical cis regulatory elements in Escherichia coli.

ABSTRACT: Recent experiments have shown that in addition to control by cis regulatory elements, the local chromosomal context of a gene also has a profound impact on its transcription. Although this chromosome-position dependent expression variation has been empirically mapped at high-resolution, the underlying causes of the variation have not been elucidated. Here, we demonstrate that 1 kb of flanking, non-coding synthetic sequences with a low frequency of guanosine and cytosine (GC) can dramatically reduce reporter expression compared to neutral and high GC-content flanks in Escherichia coli. Natural and artificial genetic context can have a similarly strong effect on reporter expression, regardless of cell growth phase or medium. Despite the strong reduction in the maximal expression level from t

SUBMITTER: Scholz SA 

PROVIDER: S-EPMC9561378 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature