Huaier suppresses pancreatic cancer progression via activating cell autophagy induced ferroptosis.

ABSTRACT:

Purpose

The anti-tumour effect of Huaier has been demonstrated in a variety of tumours. Ferroptosis is a newly identified type of programmed cell death accompanied by the accumulation of reactive oxygen species (ROS) and iron in cells and plays a key role in the therapeutic process against malignant tumours. We aimed to explore the potential therapeutic role of Huaier in pancreatic cancer and uncover the relationship between Huaier and ferroptosis.

Methods

CCK8 and colony formation assays were used to determine the proliferation of pancreatic cancer cells (PCs). The levels of cellular ROS were analysed by a fluorescence probe, and the accumulation of cellular iron was showed by Prussian blue staining. The autophagosomes and mitochondrial morphology were characterised by tran

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC9561879 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature