The Kinetic Mechanism of 3'-5' Exonucleolytic Activity of AP Endonuclease Nfo from E. coli.

ABSTRACT: Escherichia coli apurinic/apyrimidinic (AP) endonuclease Nfo is one of the key participants in DNA repair. The principal biological role of this enzyme is the recognition and hydrolysis of AP sites, which arise in DNA either as a result of the spontaneous hydrolysis of an N-glycosidic bond with intact nitrogenous bases or under the action of DNA glycosylases, which eliminate various damaged bases during base excision repair. Nfo also removes 3'-terminal blocking groups resulting from AP lyase activity of DNA glycosylases. Additionally, Nfo can hydrolyze the phosphodiester linkage on the 5' side of some damaged nucleotides on the nucleotide incision repair pathway. The function of 3'-5'-exonuclease activity of Nfo remains unclear and probably consists of participation (together with

SUBMITTER: Senchurova SI 

PROVIDER: S-EPMC9562005 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature