Evaluation of a multiplex PCR kit for detection of 17 respiratory pathogens in hospitalized patients.

ABSTRACT:

Background

Rapid pathogen identification is critical for optimizing diagnosis and treatment of infectious diseases. Multiplex polymerase chain reaction (PCR) is a sensitive, broad-spectrum molecular detection technique that is simple and rapid to perform. It is capable of simultaneously screening for multiple pathogens within a short time range. Here, we designed and evaluated a multiplex PCR kit for the identification of 17 common respiratory pathogens in clinical samples from hospitalized patients.

Methods

A total of 452 samples from hospitalized patients, including 242 respiratory and 210 non-respiratory samples, were analyzed for 13 bacteria and 4 fungi by a multiplex fluorescent PCR kit. The diagnostic performance of the kit was assessed by considering routine microbiol

SUBMITTER: Zhang C 

PROVIDER: S-EPMC9562540 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature