The Contribution of Proton-Donor pKa on Reactivity Profiles of [FeFe]-hydrogenases.

ABSTRACT: The [FeFe]-hydrogenases are enzymes that catalyze the reversible activation of H₂ coupled to the reduction-oxidation of electron carriers. Members of the different taxonomic groups of [FeFe]-hydrogenases display a wide range of preference, or bias, for H₂ oxidation or H₂ production reactions, despite sharing a common catalytic cofactor, or H-cluster. Identifying the properties that control reactivity remains an active area of investigation, and models have emerged that include diversity in the catalytic site coordination environments and compositions of electron transfer chains. The kinetics of proton-coupled electron transfer at the H-cluster might be expected to be a point of control of reactivity. To test this hypothesis, systematic changes were made to

SUBMITTER: Kisgeropoulos EC 

PROVIDER: S-EPMC9563086 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature