Functional validation of ZbFAD2 and ZbFAD3 in the alkylamide biosynthesis pathway from Zanthoxylum bungeanum Maxim.

ABSTRACT: The spicy taste and medicinal properties of Zanthoxylum bungeanum are imparted by several alkylamides. Although most studies have focused on their isolation and identification, few have reported their biosynthesis pathways. Among the differentially expressed genes (DEGs) reported in the numerous varieties of Z. bungeanum, some might contribute to alkylamide biosynthesis. However, they are not yet functionally validated. The present study explored the function of two genes, ZbFAD2 and ZbFAD3, in the alkylamide biosynthesis pathway, and their stable and transient expression in Arabidopsis thaliana and Nicotiana benthamiana were also analyzed. As compared with the wild-type (WT), the fatty acid content analysis indicated that ZbFAD2-A. thali

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9563095 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature