Gut microbiome and metabolic activity in type 1 diabetes: An analysis based on the presence of GADA.

ABSTRACT:

Objective

Type 1 diabetes (T1D) progression is affected by circulating glutamic acid decarboxylase antibody (GADA) that persist for many years. This study aimed at investigating whether and how the gut microbiome and its correlated metabolites change in T1D with the presence of GADA.

Methods

We used a radiobinding assay to measure GADA titers and identify the 49 T1D patients with GADA+ and 52 T1D patients with GADA-. The fresh feces and serum were analyzed using 16S rRNA gene sequencing and GC/MS. Then gut microbiome and serum metabolites were compared between the GADA+ patients and the GADA- patients. The association between gut microbial community and metabolites was assessed using the Spearman's rank correlation.

Results

The gut microbiome in diversity, composition

SUBMITTER: Luo S 

PROVIDER: S-EPMC9563112 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature