TPM1 mediates inflammation downstream of TREM2 via the PKA/CREB signaling pathway.

ABSTRACT:

Background

Microglia, the innate immune cells in the central nervous system, play an essential role in brain homeostasis, neuroinflammation and brain infections. Dysregulated microglia, on the other hand, are associated with neurodegenerative diseases, yet the mechanisms underlying pro-inflammatory gene expression in microglia are incompletely understood.

Methods

We investigated the role of the actin-associated protein tropomyosin 1 (TPM1) in regulating pro-inflammatory phenotype of microglia in the retina by using a combination of cell culture, immunocytochemistry, Western blot, qPCR, TUNEL, RNA sequencing and electroretinogram analysis. TREM2^-/- mice were used to investigate whether TPM1 regulated pro-inflammatory responses downstream of TREM2. To conditionally

SUBMITTER: Li R 

PROVIDER: S-EPMC9563125 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature