Transcriptome Changes in Glioma Cells Cultivated under Conditions of Neurosphere Formation.

ABSTRACT: Glioma is the most common and heterogeneous primary brain tumor. The development of a new relevant preclinical models is necessary. As research moves from cultures of adherent gliomas to a more relevant model, neurospheres, it is necessary to understand the changes that cells undergo at the transcriptome level. In the present work, we used three patient-derived gliomas and two immortalized glioblastomas, while their cultivation was carried out under adherent culture and neurosphere (NS) conditions. When comparing the transcriptomes of monolayer (ML) and NS cell cultures, we used Enrichr genes sets enrichment analysis to describe transcription factors (TFs) and the pathways involved in the formation of glioma NS. It was observed that NS formation is accompanied by the activation of five com

SUBMITTER: Vasileva NS 

PROVIDER: S-EPMC9563256 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature