Screening for α-Glucosidase-Inhibiting Saponins from Pressurized Hot Water Extracts of Quinoa Husks.

ABSTRACT: The present study extracted total saponins from quinoa husks with pressurized hot water extraction and optimized the extraction conditions. The response surface methodology (RSM) with a Box-Behnken design (BBD) was employed to investigate the effects of extraction flow rate, extraction temperature and extraction time on the extraction yield of total saponins. A maximal yield of 23.06 mg/g was obtained at conditions of 2 mL/min, 210 °C and 50 min. The constituents of the extracts were analyzed by liquid chromatography-mass spectrometry (LC-MS). A total of twenty-three compounds were identified, including five flavonoids, seventeen triterpenoid saponins and a phenolic acid. Moreover, we performed an in vitro assay for the α-glucosidase activity and found a stronger inhibitory effect o

SUBMITTER: Su R 

PROVIDER: S-EPMC9563573 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature