Differential RNA methylation analysis for MeRIP-seq data under general experimental design.

ABSTRACT:

Motivation

RNA epigenetics is an emerging field to study the post-transcriptional gene regulation. The dynamics of RNA epigenetic modification have been reported to associate with many human diseases. Recently developed high-throughput technology named Methylated RNA Immunoprecipitation Sequencing (MeRIP-seq) enables the transcriptome-wide profiling of N6-methyladenosine (m6A) modification and comparison of RNA epigenetic modifications. There are a few computational methods for the comparison of mRNA modifications under different conditions but they all suffer from serious limitations.

Results

In this work, we develop a novel statistical method to detect differentially methylated mRNA regions from MeRIP-seq data. We model the sequence count data by a hierarchical negative bi

SUBMITTER: Guo Z 

PROVIDER: S-EPMC9563684 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature