A joint use of pooling and imputation for genotyping SNPs.

ABSTRACT:

Background

Despite continuing technological advances, the cost for large-scale genotyping of a high number of samples can be prohibitive. The purpose of this study is to design a cost-saving strategy for SNP genotyping. We suggest making use of pooling, a group testing technique, to drop the amount of SNP arrays needed. We believe that this will be of the greatest importance for non-model organisms with more limited resources in terms of cost-efficient large-scale chips and high-quality reference genomes, such as application in wildlife monitoring, plant and animal breeding, but it is in essence species-agnostic. The proposed approach consists in grouping and mixing individual DNA samples into pools before testing these pools on bead-chips, such that the number of pools is less tha

SUBMITTER: Clouard C 

PROVIDER: S-EPMC9563787 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature