The molecular and functional characterization of ferritins in the hard tick Hyalomma rufipes.

ABSTRACT:

Background

The protein ferritin, which plays an important role in the maintenance of iron homeostasis, is indispensable for iron detoxification, resistance to oxidative stress and innate immunity. Ticks, which are obligate blood-sucking ectoparasites, have to deal with a large amount of iron when they take a blood meal.

Methods

Sequence analysis was undertaken using bioinformatics. A recombinant (r) expression vector, rferritin, was constructed for a prokaryotic expression system. A quantitative polymerase chain reaction platform was used to detect the spatial and temporal expression patterns of target genes and their responses to a low temperature environment. Knockdown of the ferritin genes through RNA interference was used to analyze their effects on physiological paramet

SUBMITTER: Gao Z 

PROVIDER: S-EPMC9563847 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature