Global landscape of 2-hydroxyisobutyrylation in human pancreatic cancer.

ABSTRACT: As a new type of post-translational modification (PTM), lysine 2-hydroxyisobutyrylation (K_hib) was firstly identified in histones and functioned as a regulator of transactivation in mammals. However, the role of K_hib proteins remains to be investigated. Here, we firstly identified 10,367 K_hib sites on 2,325 modified proteins in seven patients with pancreatic cancer by applying liquid chromatography with tandem mass spectrometry (LC-MS/MS) qualitative proteomics techniques. Among them, 27 K_hib-modified sites were identified in histones. Bioinformatics analysis revealed that the K_hib-modified proteins were mainly distributed in the cytoplasm and enhanced in metabolic pathways, including glycolysis/gluconeogenesis, the tricarboxylic acid

SUBMITTER: Lu Y 

PROVIDER: S-EPMC9563853 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature