Platelet-Derived Extracellular Vesicles Stimulate Migration through Partial Remodelling of the Ca²⁺ Handling Machinery in MDA-MB-231 Breast Cancer Cells.

ABSTRACT:

Background

Platelets can support cancer progression via the release of microparticles and microvesicles that enhance the migratory behaviour of recipient cancer cells. We recently showed that platelet-derived extracellular vesicles (PEVs) stimulate migration and invasiveness in highly metastatic MDA-MB-231 cells by stimulating the phosphorylation of p38 MAPK and the myosin light chain 2 (MLC2). Herein, we assessed whether the pro-migratory effect of PEVs involves the remodelling of the Ca²⁺ handling machinery, which drives MDA-MB-231 cell motility.

Methods

PEVs were isolated from human blood platelets, and Fura-2/AM Ca²⁺ imaging, RT-qPCR, and immunoblotting were exploited to assess their effect on intracellular Ca²⁺ dynamics and Ca²⁺

SUBMITTER: Vismara M 

PROVIDER: S-EPMC9564117 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature