Preservation of the Hypoxic Transcriptome in Glioblastoma Patient-Derived Cell Lines Maintained at Lowered Oxygen Tension.

ABSTRACT: Despite numerous efforts aiming to characterise glioblastoma pathology (GBM) and discover new therapeutic strategies, GBM remains one of the most challenging tumours to treat. Here we propose the optimisation of in vitro culturing of GBM patient-derived cells, namely the establishment of GBM-derived cultures and their maintenance at oxygen tension mimicking oxygenation conditions occurring within the tumour. To globally analyse cell states, we performed the transcriptome analysis of GBM patient-derived cells kept as spheroids in serum-free conditions at the reduced oxygen tension (5% O₂), cells cultured at atmospheric oxygen (20% O₂), and parental tumour. Immune cells present in the tumour were depleted, resulting in the decreased expression of the immune system and i

SUBMITTER: Gozdz A 

PROVIDER: S-EPMC9564145 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature