Synergetic collision and space separation in microfluidic chip for efficient affinity-discriminated molecular selection.

ABSTRACT: Efficient molecular selection is a prerequisite for generating molecular tools used in diagnosis, pathology, vaccinology, and therapeutics. Selection efficiency is thermodynamically highly dependent on the dissociation equilibrium that can be reached in a single round. Extreme shifting of equilibrium towards dissociation favors the retention of high-affinity ligands over those with lower affinity, thus improving the selection efficiency. We propose to synergize dual effects by deterministic lateral-displacement microfluidics, including the collision-based force effect and the two-dimensional (2D) separation-based concentration effect, to greatly shift the equilibrium. Compared with previous approaches, this system can remove more low- or moderate-affinity ligands and maintain most high-aff

SUBMITTER: Wang J 

PROVIDER: S-EPMC9565315 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature