Negatively Charged Composite Nanofibrous Hydrogel Membranes for High-Performance Protein Adsorption.

ABSTRACT: Nanofibrous materials are considered as promising candidates for fabricating high-efficiency chromatography media, which are urgently needed in protein pharmaceuticals purification and biological research, yet still face several bottlenecks. Herein, novel negatively charged composite nanofibrous hydrogel membranes (NHMs) are obtained by a facile combination of electrospinning and surface coating modification. The resulting NHMs exhibit controllable morphologies and chemical structures. Benefitting from the combined effect of the stable framework of silicon dioxide (SiO₂) nanofiber and the function layer of negatively charged hydrogel, as well as good pore connectivity among nanofibers, NHMs exhibit a high protein adsorption capacity of around 1000 mg g^-1, and are supe

SUBMITTER: Fu Q 

PROVIDER: S-EPMC9565482 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature