Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canephora.

ABSTRACT: Coffea canephora (2n = 2x = 22 chromosomes) is a species with extensive genetic diversity and desirable agronomic traits for coffee breeding programs. However, obtaining a new coffee cultivar through conventional breeding techniques may require more than 30 years of crossing cycles and selection, which hampers the effort of keeping up with market demands and rapidly proposing more resilient to climate change varieties. Although, the application of modern biotechnology tools such as precision genetic engineering technologies may enable a faster cultivar development process. Therefore, we aimed to validate the CRISPR/Cas9 system to generate mutations on a selected genotype of C. canephora, the clone 14. Embryogenic calli and a multiplex binary vector containing two sgRNAs targeting different

SUBMITTER: Casarin T 

PROVIDER: S-EPMC9568650 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature