Influence of COPD systemic environment on the myogenic function of muscle precursor cells in vitro.

ABSTRACT:

Background

Loss of muscle mass and function are well-recognized systemic manifestations of chronic obstructive pulmonary disease (COPD). Acute exacerbations, in turn, significantly contribute to upgrade these systemic comorbidities. Involvement of myogenic precursors in muscle mass maintenance and recovery is poorly understood. The aim of the present study was to investigate the effects of the vascular systemic environment from stable and exacerbated COPD patients on the myogenic behavior of human muscle precursor cells (MPC) in vitro.

Methods

Serum from healthy controls and from stable and exacerbated COPD patients (before and after Methylprednisolone treatment) was used to stimulate human MPC cultures. Proliferation analysis was assessed through BrdU incorporation assays.

SUBMITTER: Casadevall C 

PROVIDER: S-EPMC9569059 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature