Subcellular partitioning of protein kinase activity revealed by functional kinome profiling.

ABSTRACT: Protein kinases and their substrates form signaling networks partitioned across subcellular compartments to facilitate critical biological processes. While the subcellular roles of many individual kinases have been elucidated, a comprehensive assessment of the synaptic subkinome is lacking. Further, most studies of kinases focus on transcript, protein, and/or phospho-protein expression levels, providing an indirect measure of protein kinase activity. Prior work suggests that gene expression levels are not a good predictor of protein function. Thus, we assessed global serine/threonine protein kinase activity profiles in synaptosomal, nuclear, and cytosolic fractions from rat frontal cortex homogenate using peptide arrays. Comparisons made between fractions demonstrated differences in overal

SUBMITTER: Wegman-Points L 

PROVIDER: S-EPMC9569338 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature