Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model.

ABSTRACT: The hindered diffusion model postulates that the movement of a signaling molecule through an embryo is affected by tissue geometry and binding-mediated hindrance, but these effects have not been directly demonstrated in vivo. Here, we visualize extracellular movement and binding of individual molecules of the activator-inhibitor signaling pair Nodal and Lefty in live developing zebrafish embryos using reflected light-sheet microscopy. We observe that diffusion coefficients of molecules are high in extracellular cavities, whereas mobility is reduced and bound fractions are high within cell-cell interfaces. Counterintuitively, molecules nevertheless accumulate in cavities, which we attribute to the geometry of the extracellular space by agent-based simulations. We further find that Nodal has

SUBMITTER: Kuhn T 

PROVIDER: S-EPMC9569377 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature