Trypsin/Zn₃(PO₄)₂ Hybrid Nanoflowers: Controlled Synthesis and Excellent Performance as an Immobilized Enzyme.

ABSTRACT: Immobilized enzymes are a significant technological approach to retain enzyme activity and reduce enzyme catalytic cost. In this work, trypsin-incorporated Zn₃(PO₄)₂ hybrid nanoflowers were prepared via mild precipitation and coordination reactions. The controllable preparation of hybrid nanoflowers was achieved by systematically investigating the effects of the raw-material ratio, material concentration and reaction temperature on product morphology and physicochemical properties. The enzyme content of hybrid nanoflowers was about 6.5%, and the maximum specific surface area reached 68.35 m²/g. The hybrid nanoflowers exhibit excellent catalytic activity and environmental tolerance compared to free trypsin, which was attributed to the orderly accu

SUBMITTER: Wang Z 

PROVIDER: S-EPMC9569851 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature