The Defects of Epigenetic Reprogramming in Dox-Dependent Porcine-iPSCs.

ABSTRACT: Porcine-induced pluripotent stem cells (piPSCs) are of great significance to animal breeding and human medicine; however, an important problem is that the maintenance of piPSCs mainly depends on exogenous expression of pluripotent transcription factors (TFs), and germline transmission-competent piPSCs have not yet been successfully established. In this study, we explore the defect of epigenetic reprogramming during piPSCs formation, including chromatin accessibility, DNA methylation, and imprinted gene expression, with high-throughput sequencing (ATAC-seq, WGBS, RNA-seq, and Re-seq) methods. We found the somatic features were successfully silenced by connecting closed chromatin loci with downregulated genes, while DNA methylation has limited effects on somatic silence. However, the incompl

SUBMITTER: Jiang A 

PROVIDER: S-EPMC9570186 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature