Nuclear Functions of KaeA, a Subunit of the KEOPS Complex in Aspergillus nidulans.

ABSTRACT: Kae1 is a subunit of the highly evolutionarily conserved KEOPS/EKC complex, which is involved in universal (t6A₃₇) tRNA modification. Several reports have discussed the participation of this complex in transcription regulation in yeast and human cells, including our previous observations of KaeA, an Aspergillus nidulans homologue of Kae1p. The aim of this project was to confirm the role of KaeA in transcription, employing high-throughput transcriptomic (RNA-Seq and ChIP-Seq) and proteomic (LC-MS) analysis. We confirmed that KaeA is a subunit of the KEOPS complex in A. nidulans. An analysis of kaeA19 and kaeA25 mutants showed that, although the (t6A₃₇) tRNA modification is unaffected in both mutants, they reveal significantly altered transcr

SUBMITTER: Gawlik J 

PROVIDER: S-EPMC9570407 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature