Control of Grain Shape and Size in Rice by Two Functional Alleles of OsPUB3 in Varied Genetic Background.

ABSTRACT: Grain shape and size are key determinants of grain appearance quality and yield in rice. In our previous study, a grain shape QTL, qGS1-35.2, was fine-mapped using near-isogenic lines (NILs) derived from a cross between Zhenshan 97 (ZS97) and Milyang 46 (MY46). One annotated gene, OsPUB3, was found to be the most likely candidate gene. Here, knockout and overexpression experiments were performed to investigate the effects of OsPUB3 on grain shape and size. Four traits were tested, including grain length, grain width, grain weight, and the ratio of grain length to width. Knockout of OsPUB3 in NIL^ZS97, NIL^MY46, and another rice cultivar carrying the OsPUB3^MY46 allele all caused decreases in grain width and weight and increas

SUBMITTER: Li ZH 

PROVIDER: S-EPMC9571118 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature