Enhancement of linalool production in Saccharomyces cerevisiae by utilizing isopentenol utilization pathway.

ABSTRACT:

Background

Linalool is a monoterpenoid, also a vital silvichemical with commercial applications in cosmetics, flavoring ingredients, and medicines. Regulation of mevalonate (MVA) pathway metabolic flux is a common strategy to engineer Saccharomyces cerevisiae for efficient linalool production. However, metabolic regulation of the MVA pathway is complex and involves competition for central carbon metabolism, resulting in limited contents of target metabolites.

Results

In this study, first, a truncated linalool synthase (t26AaLS1) from Actinidia arguta was selected for the production of linalool in S. cerevisiae. To simplify the complexity of the metabolic regulation of the MVA pathway and increase the flux of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DM

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9571491 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature