Silica/Proteoliposomal Nanocomposite as a Potential Platform for Ion Channel Studies.

ABSTRACT: The nanostructuration of solid matrices with lipid nanoparticles containing membrane proteins is a promising tool for the development of high-throughput screening devices. Here, sol-gel silica-derived nanocomposites loaded with liposome-reconstituted KcsA, a prokaryotic potassium channel, have been synthesized. The conformational and functional stability of these lipid nanoparticles before and after sol-gel immobilization have been characterized by using dynamic light scattering, and steady-state and time-resolved fluorescence spectroscopy methods. The lipid-reconstituted KcsA channel entrapped in the sol-gel matrix retained the conformational and stability changes induced by the presence of blocking or permeant cations in the buffer (associated with the conformation of the selectivity fil

SUBMITTER: Esquembre R 

PROVIDER: S-EPMC9571612 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature