A New Set of Golden-Gate-Based Organelle Marker Plasmids for Colocalization Studies in Plants.

ABSTRACT: In vivo localization of proteins using fluorescence-based approaches by fusion of the protein of interest (POI) to a fluorescent protein is a cost- and time-effective tool to gain insights into its physiological function in a plant cell. Determining the proper localization, however, requires the co-expression of defined organelle markers (OM). Several marker sets are available but, so far, the procedure requires successful co-transformation of POI and OM into the same cell and/or several cloning steps. We developed a set of vectors containing markers for basic cell organelles that enables the insertion of the gene of interest (GOI) by a single cloning step using the Golden Gate cloning approach and resulting in POI-GFP fusions. The set includes markers for plasma membrane, tonoplast, nucle

SUBMITTER: Stellmach H 

PROVIDER: S-EPMC9572143 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature