Decreased Tertiary Lymphoid Structures in Lung Adenocarcinomas with ALK Rearrangements.

ABSTRACT:

Purpose

This study sought to characterize the tumor immune microenvironment (TIME) of lung adenocarcinomas with ALK rearrangements (ALK+ LUAD), which responds poorly to immune checkpoint inhibitors (ICIs) therapy.

Materials and methods

Immune score evaluation and immunohistochemical (IHC) validation of B cells, cytotoxic, helper, regulatory T cells, dendritic cells, and tumor-associated macrophages were performed on the TCGA cohort and the whole tissue sections of our matched surgical samples, respectively, between ALK+ and ALK- LUAD. The formation and spatial organization of TLS, intra- and extra-TLS immune cell features, and tumor PD-L1 expression were analyzed independently.

Results

Immune scores and TLS-signature gene levels were found to be lower in ALK+ TCGA LUA

SUBMITTER: Zou Y 

PROVIDER: S-EPMC9572246 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature