Myo-Inositol Moderates Glucose-Induced Effects on Human Placental ¹³C-Arachidonic Acid Metabolism.

ABSTRACT: Maternal hyperglycemia is associated with disrupted transplacental arachidonic acid (AA) supply and eicosanoid synthesis, which contribute to adverse pregnancy outcomes. Since placental inositol is lowered with increasing glycemia, and since myo-inositol appears a promising intervention for gestational diabetes, we hypothesized that myo-inositol might rectify glucose-induced perturbations in placental AA metabolism. Term placental explants (n = 19) from women who underwent a mid-gestation oral glucose-tolerance-test were cultured with ¹³C-AA for 48 h in media containing glucose (5, 10 or 17 mM) and myo-inositol (0.3 or 60 µM). Newly synthesized ¹³C-AA-lipids were quantified by liquid-chromatography-mass-spectrometry. Increasing maternal fasting glycemia was ass

SUBMITTER: Watkins OC 

PROVIDER: S-EPMC9572372 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature