3D Polymer Architectures for the Identification of Optimal Dimensions for Cellular Growth of 3D Cellular Models.

ABSTRACT: Organ-on-chips and scaffolds for tissue engineering are vital assay tools for pre-clinical testing and prediction of human response to drugs and toxins, while providing an ethical sound replacement for animal testing. A success criterion for these models is the ability to have structural parameters for optimized performance. Here we show that two-photon polymerization fabrication can create 3D test platforms, where scaffold parameters can be directly analyzed by their effects on cell growth and movement. We design and fabricate a 3D grid structure, consisting of wall structures with niches of various dimensions for probing cell attachment and movement, while providing easy access for fluorescence imaging. The 3D structures are fabricated from bio-compatible polymer SZ2080 and subsequently

SUBMITTER: Maibohm C 

PROVIDER: S-EPMC9572445 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature