Dataset Information

Fluorine-Nitrogen-Codoped Carbon Dots as Fluorescent Switch Probes for Selective Fe(III) and Ascorbic Acid Sensing in Living Cells.

ABSTRACT: High-quality fluorescent probes based on carbon dots (CDs) have promising applications in many fields owing to their good stability, low toxicity, high quantum yield, and low raw material price. The fluorine- and nitrogen-doped fluorescent CDs (NFCDs) with blue fluorescence was successfully synthesized using 3-aminophenol and 2,4-difluorobenzoic acid as the raw material by the hydrothermal method. The NFCDs as probe can be used to directly and indirectly detect Fe³⁺ (detection range: 0.1-150 μM and detection limit: 0.14 μM) and ascorbic acid (AA) (detection range: 10-80 μM and detection limit: 0.11 μM). The NFCDs-based probe shows exceptional selectivity and strong anti-interference for Fe³⁺ and ascorbic acid (AA). In addition, we examined the response of NFCDs to Fe³⁺ and AA in living cells, which showed that the timely use of AA can reduce the effects of iron poisoning. This has important biological significance. This means that using NFCDs as fluorescent probes is beneficial for Fe³⁺ and AA detection and observing their dynamic changes in living cells. Thus, this work may contribute to the study of Fe³⁺- and AA-related diseases.

SUBMITTER: Ye S

PROVIDER: S-EPMC9572543 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Fluorine-Nitrogen-Codoped Carbon Dots as Fluorescent Switch Probes for Selective Fe(III) and Ascorbic Acid Sensing in Living Cells.

Ye Shuai S Zhang Mingming M Guo Jiaqing J Yu Xiantong X Song Jun J Zeng Pengju P Qu Junle J Chen Yue Y Li Hao H

Molecules (Basel, Switzerland) 20220920 19

High-quality fluorescent probes based on carbon dots (CDs) have promising applications in many fields owing to their good stability, low toxicity, high quantum yield, and low raw material price. The fluorine- and nitrogen-doped fluorescent CDs (NFCDs) with blue fluorescence was successfully synthesized using 3-aminophenol and 2,4-difluorobenzoic acid as the raw material by the hydrothermal method. The NFCDs as probe can be used to directly and indirectly detect Fe<sup>3+</sup> (detection range: ...[more]

PMID: 36234694

Similar Datasets

Project description:Fluorescent carbon dots (CDs) have been identified as potential nanosensors and attracted tremendous research interests in wide areas including anti-counterfeiting, environmental and biological sensing and imaging in considering of the attractive optical properties. In this work, we present a CDs based fluorescent sensor from polyvinylpyrrolidone, citric acid, and methionine as precursors by hydrothermal approach. The selective quantifying of Fe3+ and ascorbic acid (AA) are based on the fluorescent on-off-on process, in which the fluorescent quenching is induced by the coordination of the Fe3+ on the surface of the CDs, while the fluorescence recovery is mainly attributed to redox reaction between Fe3+ and AA, breaking the coordination and bringing the fluorescence back. Inspired by the good water solubility and biocompatibility, significant photostability, superior photobleaching resistance as well as high selectivity, sensitivity, and interference immunity, which are constructed mainly from the N,S-doping and methionine surface functionalization, the CDs have not only been employed as fluorescence ink in multiple anti-counterfeiting printing and confidential document writing or transmitting, but also been developed as promising fluorescence sensors in solution and solid by CDs doped test strips and hydrogels for effectively monitoring and removing of Fe3+ and AA in environmental aqueous solution. The CDs have been also implemented as effective diagnostic candidates for imaging and tracking of Fe3+ and AA in living cells, accelerating the understanding of their function and importance in related biological processes for the prevention and treatment specific diseases.Electronic supplementary materialSupplementary material (fluorescence spectra: UV and Xe irradiation, TG, thermo stability, ionic strength, relationship between fluorescence responses at different concentrations of Fe3+ and AA, reaction time-dependent fluorescent responses; XPS spectra of CDs + Fe3+ and Fe3+@CDs + AA; structural characterization; equations about fluorescence lifetime, quantum yield and LOD; comparison of the CDs for the detection of Fe3+ and AA with reported methods; detection of Fe3+ and AA in real samples; absorption of Fe3+ in environmental samples and MTT assay results) is available in the online version of this article at 10.1007/s12274-022-5107-7.

Dataset Information

Fluorine-Nitrogen-Codoped Carbon Dots as Fluorescent Switch Probes for Selective Fe(III) and Ascorbic Acid Sensing in Living Cells.

Publications

Fluorine-Nitrogen-Codoped Carbon Dots as Fluorescent Switch Probes for Selective Fe(III) and Ascorbic Acid Sensing in Living Cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets