κ-Carrageenan Hydrogel as a Matrix for Therapeutic Enzyme Immobilization.

ABSTRACT: During the last few decades, polysaccharide hydrogels attract more and more attention as therapeutic protein delivery systems due to their biocompatibility and the simplicity of the biodegradation of natural polymers. The protein retention by and release from the polysaccharide gel network is regulated by geometry and physical interactions of protein with the matrix. In the present work, we studied the molecular details of interactions between κ-carrageenan and three lipases, namely the lipases from Candida rugosa, Mucor javanicus, and Rhizomucor miehei-which differ in their size and net charge-upon protein immobilization in microparticles of polysaccharide gel. The kinetics of protein release revealed the different capability of κ-carrageenan to retain lipases, which

SUBMITTER: Makshakova ON 

PROVIDER: S-EPMC9573024 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature