Ex vivo engineered human plasma cells exhibit robust protein secretion and long-term engraftment in vivo.

ABSTRACT: Due to their unique longevity and capacity to secrete high levels of protein, plasma B cells have the potential to be used as a cell therapy for protein replacement. Here, we show that ex vivo engineered human plasma cells exhibit single-cell RNA profiles, scanning electron micrograph ultrastructural features, and in vivo homing capacity of long-lived plasma cells. After transferring human plasma cells to immunodeficient mice in the presence of the human cytokines BAFF and IL-6, we observe increases in retention of plasma cells in the bone marrow, with engraftment exceeding a year. The most profound in vivo effects of human IL-6 are observed within 20 days of transfer and could be explained by decreased apoptosis in newly differentiated plasma cells. Collectively, these results show that e

SUBMITTER: Cheng RY 

PROVIDER: S-EPMC9573882 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature