Exosomal RNF157 mRNA from prostate cancer cells contributes to M2 macrophage polarization through destabilizing HDAC1

ABSTRACT:

Background

Exosomes have been identified to mediate the transmission of RNAs among different cells in tumor microenvironment, thus affecting the progression of different diseases. However, exosomal messenger RNAs (mRNAs) have been rarely explored. RNF157 mRNA has been found to be up-regulated in PCa patients’ exosomes, but the role of exosomal RNF157 mRNA in PCa development remains unclear.

Methods

Online databases were utilized for predicting gene expression and binding correlation between different factors. RT-qPCR and western blot assays were respectively done to analyze RNA and protein expressions. Flow cytometry analysis was implemented to analyze M2 polarization.

Results

RNF157 expression was high in PCa tissues and cells. M2 polarization of macrophages was

SUBMITTER: Guan H 

PROVIDER: S-EPMC9573993 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature