A tunable physiomimetic stretch system evaluated with precision cut lung slices and recellularized human lung scaffolds.

ABSTRACT: Breathing exposes lung cells to continual mechanical stimuli, which is part of the microenvironmental signals directing cellular functions together with the extracellular matrix (ECM). Therefore, developing systems that incorporate both stimuli is urgent to fully understand cell behavior. This study aims to introduce a novel in vitro culture methodology combining a cyclic stretch that simulates in vivo breathing with 3D cell culture platforms in the form of decellularized lung slices (DLS) and precision cut lung slices (PCLS). To this end, we have constructed a device that mimics the amplitudes and frequencies of distensions seen in the breathing human lung. For its validation, we cultured H441 lung epithelial cells in human DLS exposed to 16 stretch cycles per minute with a

SUBMITTER: Rosmark O 

PROVIDER: S-EPMC9574011 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature