Comparative study on the microbiota of colostrum and nipple skin from lactating mothers separated from their newborn at birth in China.

ABSTRACT: Increasing studies have found breast milk (BM) contains its own microbiota. However, the route through which microbes enter the BM is still unclear. In order to verify the entero-mammary pathway of BM, we designed a rigorous study that prevented oral bacteria from contaminating the breast and nipple skin (NS) during baby nursing. Thirty-one healthy, postpartum mothers living in southern China who were immediately separated from their newborn after delivery were enrolled in this study. Using an aseptic protocol for sampling, sterile water was used to wash the NS and was then collected. Then the first drop of BM was discarded and colostrum was collected manually. Amplicon sequencing was performed targeting the V3-V4 region of the bacterial 16S rRNA gene, and the differences between the micro

SUBMITTER: Du Y 

PROVIDER: S-EPMC9574262 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature