Validation of a new strategy for the identification of SARS-CoV-2 variants by sequencing the spike gene by Sanger.

ABSTRACT:

Introduction

The emergence of multiple variants of SARS-CoV-2 during the COVID-19 pandemic is of great world concern. Until now, their analysis has mainly focused on next-generation sequencing. However, this technique is expensive and requires sophisticated equipment, long processing times, and highly qualified technical personnel with experience in bioinformatics. To contribute to the analysis of variants of interest and variants of concern, increase the diagnostic capacity, and process samples to carry out genomic surveillance, we propose a quick and easy methodology to apply, based on Sanger sequencing of 3 gene fragments that code for protein spike.

Methods

Fifteen positive samples for SARS-CoV-2 with a cycle threshold below 25 were sequenced by Sanger and next-generatio

SUBMITTER: Murillo E 

PROVIDER: S-EPMC9574460 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature