Ontology highlight
ABSTRACT:
SUBMITTER: Fatima N
PROVIDER: S-EPMC9574506 | biostudies-literature | 2022 Nov
REPOSITORIES: biostudies-literature
Fatima Naureen N Rehman Abdul A Bukhari DilAra Abbas DA
Saudi journal of biological sciences 20220927 11
The current investigation describes the isolation and characterization of toxic <i>Bt</i>. local isolates harboring 99% homology with <i>Bti.</i> prototoxin <i>Bacillus thuringiensis</i> (AXJ97553.1 and novel OUB27301.1) which contains full length <i>cry11</i> gene (1.9 kb). Initially, it was cloned in pTZ57R/T and then sub-cloned in pET30a(+) for expression. The optimized conditions for good expression were found 1 mM IPTG, 3.5-4 h incubation time, and 37 °C. Toxicological assays were determine ...[more]