In vivo expansion of a CD9⁺ decidual-like NK cell subset following autologous hematopoietic stem cell transplantation.

ABSTRACT: Autologous hematopoietic stem cell transplantation (autoHSCT) is a treatment option for hematological disorders and pediatric solid tumors. After an autoHSCT, natural killer (NK) cells are the first lymphocyte subset returning to normal levels. To uncover global changes during NK cell reconstitution after autoHSCT, we performed RNA-sequencing on NK cells before and after autoHSCT. Results showed profound changes in the gene expression profile of NK cells immediately after autoHSCT. Several biological processes including cell cycle, DNA replication and the mevalonate pathway were enriched. Significantly, we observed that following autoHSCT, NK cells acquired a decidual-like gene expression profile, including the expression of CD9. By using multiparametric flow cytometry, we confirmed the ex

SUBMITTER: Orrantia A 

PROVIDER: S-EPMC9574507 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature