Quantitative cerebrovascular reactivity MRI in mice using acetazolamide challenge.

ABSTRACT:

Purpose

To develop a quantitative MRI method to estimate cerebrovascular reactivity (CVR) in mice.

Methods

We described an MRI procedure to measure cerebral vasodilatory response to acetazolamide (ACZ), a vasoactive agent previously used in human clinical imaging. Vascular response was determined by cerebral blood flow (CBF) measured with phase-contrast or pseudo-continuous arterial spin labeling MRI. Vasodilatory input intensity was determined by plasma ACZ level using high-performance liquid chromatography. We verified the source of the CVR MRI signal by comparing ACZ injection to phosphate-buffered saline injection and noninjection experiments. Dose dependence and feasibility of regional CVR measurement were also investigated.

Results

Cerebral blood flow revealed a

SUBMITTER: Wei Z 

PROVIDER: S-EPMC9574885 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature