Quantification of cell-free circulating mitochondrial DNA copy number variation in hepatocellular carcinoma.

ABSTRACT:

Objective

Hepatocellular carcinoma is the most common primary malignant liver tumor. Mitochondrial DNA copy number has been shown to be associated with various malignancies. However, there has not been any study on the absolute quantification of mtDNA copy number in hepatocellular carcinoma. The aim of this study was to develop a new method for absolute quantification of mtDNA copy number and to relatively quantify the variations in the mtDNA copy number in hepatocellular carcinoma patients in comparison with healthy individuals.

Methods

Venous blood samples were collected from both hepatocellular carcinoma patients (34) and healthy individuals (34). Circulating cell-free DNAs were isolated and the relative quantification of mtDNA copy number variation was determined using q

SUBMITTER: Yalcınkaya B 

PROVIDER: S-EPMC9575012 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature