Improving culture initiation of mature oak shoots through use of silver thiosulfate.

ABSTRACT:

Premise

Of the approximately 430 species of oaks (Quercus spp.) that have been assessed, 31% are threatened with extinction and in need of safeguarding. However, oak seeds cannot be seed banked, and thus rely on alternative strategies such as in vitro culture for ex situ conservation. One challenge to this approach is low culture initiation rates. Our objective was to identify factors that may improve the establishment of shoot cultures in vitro using new growth collected from mature trees.

Methods

Shoot cuttings were harvested from individuals of five different oak species (Q. alba, Q. bicolor, Q. macrocarpa, Q. muehlenbergii, and Q. palustris). Shoots were cultured onto medium with or without 50 µM silver thiosulfate (STS), a known

SUBMITTER: Winkeljohn M 

PROVIDER: S-EPMC9575081 | biostudies-literature | 2022 Sep-Oct

REPOSITORIES: biostudies-literature